Lab tool

Peptide reconstitution calculator

Enter the vial's milligrams, the bacteriostatic water to add and the target dose per draw. The tool computes concentration, volume per dose and the units in an insulin U-100 syringe.

Syringe
5 mg/ml
5,000 mcg/ml
Concentration
10 U
In the syringe (U-100)
0.1 ml
Volume per dose
20
Doses per vial

05010010⚠︎ 10 U

The link opens the calculator with these same values.

Calculation tool for laboratory planning (RUO). An insulin U-100 syringe has 100 units = 1 ml. Laboratory reference, not medical advice.

How to reconstitute

Step by step, with your numbers

Your numbers

10 mg vial · 2 ml of water · 0.5 mg dose · 1 ml syringe

Change the numbers

Step 1 of 4 Get what you need

Your vial, a vial of bacteriostatic water (sold separately), a 1 ml U-100 insulin syringe — ask for it exactly like that at the pharmacy — and alcohol wipes.

Step 2 of 4 Measure the water

The 10 mg vial takes 2 ml of water: 2 full draws to 100. Flip off the plastic cap (the rubber stopper stays on), wipe it with alcohol and let the water run down the inside wall.

Step 3 of 4 Swirl gently, never shake

Swirl the vial slowly until it turns clear — never shake it; some take a few minutes. Then it goes in the fridge (2–8 °C), away from light, and is used within 12 weeks — the limit is mainly to keep bacteria out each time the stopper is pierced.

Step 4 of 4 Fill to the 10 mark

The numbers printed on the syringe are units (U): 100 U = 1 ml. Your dose of 0.5 mg is 0.1 ml — draw up to the 10 mark.

Summary Done — you have the essentials

Your 10 mg vial yields 20 complete doses of 0.5 mg.

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Start from a vial in the catalog

Each button loads that presentation's real milligrams and a common water volume. You can adjust them afterwards.

See the full catalog →

Concentration of our vials

What concentration you get depending on the water you add, for every presentation in the catalog.

Vial0.5 ml1 ml2 ml3 ml
Retatrutide · 5 mg10 mg/ml5 mg/ml2.5 mg/ml1.67 mg/ml
Retatrutide · 15 mg30 mg/ml15 mg/ml7.5 mg/ml5 mg/ml
Retatrutide · 30 mg60 mg/ml30 mg/ml15 mg/ml10 mg/ml
Tirzepatide · 20 mg40 mg/ml20 mg/ml10 mg/ml6.67 mg/ml
Tesamorelin · 10 mg20 mg/ml10 mg/ml5 mg/ml3.33 mg/ml
MOTS-c · 40 mg80 mg/ml40 mg/ml20 mg/ml13.33 mg/ml
BPC-157 + TB-500 · 10 mg20 mg/ml10 mg/ml5 mg/ml3.33 mg/ml
Ipamorelin + CJC-1295 · 10 mg20 mg/ml10 mg/ml5 mg/ml3.33 mg/ml
GHK-Cu · 50 mg100 mg/ml50 mg/ml25 mg/ml16.67 mg/ml
NAD+ · 500 mg1,000 mg/ml500 mg/ml250 mg/ml166.67 mg/ml
SEMAX · 10 mg20 mg/ml10 mg/ml5 mg/ml3.33 mg/ml
PT-141 · 10 mg20 mg/ml10 mg/ml5 mg/ml3.33 mg/ml

U-100 syringe equivalence

How many units to draw depending on the concentration you prepared and the mass you want.

Concentration0.1 mg0.25 mg0.5 mg1 mg2 mg
1 mg/ml10 U25 U50 U100 U200 U
2 mg/ml5 U12.5 U25 U50 U100 U
2.5 mg/ml4 U10 U20 U40 U80 U
5 mg/ml2 U5 U10 U20 U40 U
10 mg/ml1 U2.5 U5 U10 U20 U
20 mg/ml0.5 U1.3 U2.5 U5 U10 U

Milligrams and micrograms

There is a factor of a thousand between a milligram and a microgram. It is the most common mistake when reconstituting, and the most expensive: getting the unit wrong does not shift the result slightly, it multiplies or divides it by a thousand.

Vials are labelled in milligrams and much of the research literature speaks in micrograms. A 10 mg vial holds 10,000 mcg. If a protocol says 250 mcg, that is 0.25 mg — one fortieth of the vial, not a quarter. The calculator asks for the dose in milligrams and shows the microgram equivalent underneath, so you never have to do that conversion in your head.

Reading an insulin syringe

A U-100 syringe has 100 units per millilitre, so each unit is 0.01 ml. To convert: divide the mass you want by the concentration in mg/ml and multiply by 100.

What changes between syringes is barrel capacity, and with it precision — and the value of each line: on 0.3 and 0.5 ml syringes it is usually 1 unit (some 0.3 ml ones mark half units), on a 1 ml syringe usually 2 units, so go by the printed numbers, not by counting lines. 40 units fit comfortably in a 1 ml syringe and do not fit at all in a 0.3 ml one — that is why the selector above matters. The tool warns you when the volume does not fit the barrel you picked.

Frequently asked questions

How many units is 0.5 mg?

It depends on the concentration you prepared, not on the peptide. At 2.5 mg/ml it is 20 units; at 5 mg/ml, 10; at 10 mg/ml, 5. The formula: mass in mg ÷ concentration in mg/ml × 100.

Does it work for non U-100 syringes?

The calculator assumes U-100, the standard for human insulin syringes. A veterinary U-40 syringe holds 40 units per millilitre instead of 100, so the units are not equivalent. Check what is printed on the barrel.