Reference protocol

Reference protocol: Ipamorelin + CJC-1295

This blend pairs a GHRH analog (CJC-1295 without DAC) with a selective secretagogue (Ipamorelin): two distinct pathways that amplify the same growth hormone pulse. The No-DAC version is dosed daily because its pulse is short, like the physiological one.

The reference for the Ipamorelin + CJC-1295 No-DAC blend (5 mg + 5 mg) is one daily dose at bedtime, after 2 to 3 hours of fasting: start at 240 mcg and increase gradually to 600 mcg within a 12 to 16 week cycle. With 2.5 ml of water, 240 mcg is 6 units.

Updated August 25, 20267 min read

First time? Follow the step-by-step guide

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First time? Start here

Your Ipamorelin + CJC-1295 vial arrives as a dry powder: you mix it with a special water — that step is called reconstituting — and measure each dose with an insulin syringe. Follow the steps with the numbers for your case.

Which case is yours?

Start at 240 mcg (6 U) and titrate toward 600 within the cycle.

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Step 1 of 6 Get what you need

Your vial, a vial of bacteriostatic water (sold separately), a 1 ml U-100 insulin syringe — ask for it exactly like that at the pharmacy — and alcohol wipes.

Step 2 of 6 Measure the water

The 10 mg vial takes 2.5 ml of water: 2 full draws to 100 and one up to the 50 mark. Flip off the plastic cap (the rubber stopper stays on), wipe it with alcohol and let the water run down the inside wall.

Step 3 of 6 Swirl gently, never shake

Swirl the vial slowly until it turns clear — never shake it; some take a few minutes. Then it goes in the fridge (2–8 °C) and is used within 12 weeks — the limit is mainly to keep bacteria out each time the stopper is pierced. Unmixed, keep it refrigerated from the day it arrives too.

Step 4 of 6 Fill to the 6 mark

The numbers printed on the syringe are units (U): 100 U = 1 ml. Your dose of 240 mcg is 0.06 ml — draw up to the 6 mark.

Step 5 of 6 Inject under the skin

Belly area, two fingers away from the navel: alcohol, pinch the skin, insert the full needle and inject slowly. Switch sides each time and use a new syringe.

Step 6 of 6 Your schedule

Used right before bed, 2–3 hours after your last meal.

Cycle: 12–16 weeks · 8–16 week washout

240 mcg your dose6 Ugradual
step up gradually
up to 600 mcg15 U

Summary Done — you have the essentials

Your 10 mg vial yields 41 complete doses of 240 mcg (≈ 6 weeks).

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Quick dictionary — the words that stop beginners

Reconstitute: mixing the vial's powder with the special water so it becomes a liquid you can measure.
Bacteriostatic water: the special water for that mix (regular water will not do); sold separately — one vial covers several mixes.
Unit (U): the insulin syringe's measure: 100 units = 1 ml. Go by the printed numbers, not by counting lines (on a 1 ml syringe each line is usually 2 U).
U-100 syringe: the standard 1 ml insulin syringe, numbered from 0 to 100 units; sold at any pharmacy.
mg and mcg: milligrams and micrograms: 1 mg = 1,000 mcg. Mind the factor of one thousand when reading a dose.
Subcutaneous: an injection under the skin (not into muscle or vein), usually in the belly area.
Cycle and washout: using the product for a period and then stopping completely for some weeks; when resuming you start again from the starting dose.

Quick facts

Parameter	Reference
Frequency	1 daily dose, 5–7 days per week
Time of day	Right before bed, after 2–3 h of fasting (alternative: upon waking, before breakfast)
Escalation	Standard: 240 → 600 mcg · Intensive: 480 → 1,200 mcg, gradual within the cycle
Cycle	12–16 weeks · 8–16 week washout
Technique	Subcutaneous injection, SLOW

Why this blend takes 2.5 ml of water

Our house 1-to-1 rule would leave this 10 mg vial at 10 mg/ml, where the starting dose of 240 mcg is 2.4 units: a mark too fine to read with confidence on any syringe. For this product we recommend 2.5 ml of bacteriostatic water — it lands at 4 mg/ml — and the same doses become thick, legible marks: 240 mcg is 6 units.

Reconstitution and syringe units

For this product we recommend 2.5 ml of bacteriostatic water for the 10 mg vial: it sits at 4 mg/ml (this page explains why).

Dose

The buttons follow the protocol's dose steps; the starting dose comes pre-selected.

Fill the syringe up to the 6 mark

6 U0.06 ml · in a U-100 syringe

The syringe: a 1 ml U-100 insulin syringe (numbered 0 to 100 U = 1 ml), sold at any pharmacy. Go by the printed numbers, not by counting lines.

The 10 mg vial covers 41 complete doses (≈ 6 weeks)

Open this calculation in the calculator

The protocol week

Daily at bedtime · 5–7 days per week

See the full dose-to-units table

Dose	U-100 syringe	Volume	Doses per vial
240 mcg	6 U	0.06 ml	41
300 mcg	7.5 U	0.08 ml	33
480 mcg	12 U	0.12 ml	20
600 mcg	15 U	0.15 ml	16
1,200 mcg	30 U	0.3 ml	8

Units refer to an insulin U-100 syringe (100 units = 1 ml). Tap a figure to open it in the calculator. The last column shows how many complete doses the vial yields.

Timing and technique matter

Before bed, after 2–3 hours without food: the GH pulse it stimulates occurs during deep sleep, and a recent meal — the elevated insulin — blunts it. Do not eat after the dose.
The documented alternative is upon waking, before breakfast, if the nighttime dose does not fit your routine.
Inject SLOWLY: rapid administration produces warmth, flushing, and a pins-and-needles sensation on the skin. It is transient, but almost entirely avoided by injecting slowly.

What No-DAC means

CJC-1295 exists in two versions. The DAC version binds to albumin and keeps GH elevated for days with a single dose — at the cost of the documented risk of desensitization from constant stimulation. The version without DAC (the one in our blend) has a half-life of about 30 minutes and produces a 2 to 3 hour pulse that mimics natural secretion: that is why it is dosed daily and why timing weighs so heavily on the result.

Documented precautions

Reported effects: fluid retention, swelling, increased appetite, mild fatigue after the dose, headache, flushing or warmth when injecting fast.
It can transiently reduce insulin sensitivity: caution and monitoring with glucose alterations or glucose-lowering medication.
Corticosteroids at high or chronic doses blunt GH release and reduce the blend's effectiveness.
Contraindicated with active cancer (including pituitary adenomas), untreated hypothyroidism, pregnancy, and breastfeeding.

In our catalog

The 10 mg blend (5 + 5) is on the Ipamorelin + CJC-1295 product page. In documented research practice it is combined with Tesamorelin for synergistic GH elevation, and with BPC-157 + TB-500 for connective tissue recovery.

Frequently asked questions

Why at bedtime?

The main growth hormone pulse occurs during deep sleep, and this blend amplifies it. Dosing right before bed, after 2 to 3 hours of fasting, aligns the stimulus with that pulse; the insulin from a recent meal blunts it.

Can I still use the 1-to-1 rule (1 ml)?

You can, but 240 mcg comes out to 2.4 units: a mark that is hard to read precisely. With 2.5 ml the same dose is 6 units. The calculator shows you both variants instantly.

How long does the vial last?

At 240 mcg daily, 41 complete doses — about six weeks; at 600 mcg, 16 doses. A 12–16 week cycle with full escalation requires 3 to 5 vials depending on the dose ceiling.

Is our CJC-1295 with or without DAC?

Without DAC: 5 mg of CJC-1295 No-DAC and 5 mg of Ipamorelin per vial. That is why the protocol is daily and strictly timed; a DAC blend would be dosed completely differently.

Reference protocols compiled from documented research practice and adapted to our formats. Research-context documentation (RUO): not medical advice, not a prescription, and no substitute for professional evaluation.