Reference protocol: Tesamorelin
Tesamorelin is a GHRH analog and its protocol punishes impatience: the effect is cumulative, and the documentation agrees that anything under 12 weeks is wasted time. Timing matters as much as the dose.
The reference for tesamorelin is 1 mg per day —10 units at 10 mg/ml—, five consecutive days followed by two rest days, at night and at least 90 minutes after the last meal, for 12 to 16 weeks. Shorter cycles show no results in documented research practice.
View product & buy →Which case is yours?
1 mg per night (10 U) — the reference for most cases.
First time? Start here
Your Tesamorelin vial arrives with the compound as a dry powder. Before using it you mix it with a special water — that step is called reconstitution — and measure each dose with an insulin syringe. Here is everything you need, in order.
- Get what you need: your vial, a vial of bacteriostatic water (not included — it is the special mixing water), a 1 ml U-100 insulin syringe — ask for it exactly like that at any pharmacy — and alcohol wipes.
- Mix the powder with the water (reconstitute): the vial is never uncapped — the needle goes through the rubber stopper. Add 1 ml of water letting it run down the inside wall, then swirl gently — never shake; it turns clear in a few minutes (some compounds take longer). Measure the water with the same insulin syringe: one full draw to the top is exactly 1 ml — do not repeat it. Once mixed, the vial goes in the fridge and lasts about 4 weeks; unmixed, also keep it refrigerated from the day it arrives.
- Fill the syringe up to the 10 mark: every small line on the insulin syringe is 1 unit (U). That mark equals the starting dose (1 mg); if your dose is different, pick it in the panel below and the number updates. And note: several protocols raise the dose after the first weeks — the dose steps further down tell you when your mark changes.
- Inject under the skin and you are done: belly area (two fingers away from the navel), skin cleaned with alcohol; pinch the skin, insert the full needle and inject slowly. Switch sides each time and use a new syringe for every injection. Used each night (5 nights on, 2 off), at least 90 minutes after eating.
A question about your case? Message us on WhatsApp and we will sort it out with you.
Quick dictionary — the words that stop beginners
- Reconstitute: mixing the vial's powder with the special water so it becomes a liquid you can measure.
- Bacteriostatic water: the special water for that mix (regular water will not do); sold separately — one vial covers several mixes.
- Unit (U): each small line on the insulin syringe. 100 units = 1 ml.
- U-100 syringe: the standard 1 ml insulin syringe with 100 marks; sold at any pharmacy.
- mg and mcg: milligrams and micrograms: 1 mg = 1,000 mcg. Mind the factor of one thousand when reading a dose.
- Subcutaneous: an injection under the skin (not into muscle or vein), usually in the belly area.
- Cycle and washout: using the product for a period and then stopping completely for some weeks; when resuming you start again from the starting dose.
Quick facts
| Parameter | Reference |
|---|---|
| Frequency | 1 daily dose, 5 consecutive days + 2 rest days |
| Time of day | At night, ≥90 minutes after the last meal |
| Dose | Standard 1 mg · documented maximum 2 mg |
| Cycle | 12–16 weeks · 4–8 week washout |
| Documented route | Subcutaneous injection |
Reconstitution and syringe units
Our 10 mg vial is reconstituted with 1 ml of bacteriostatic water: it sits at 10 mg/ml.
The buttons follow the protocol's dose steps; the starting dose comes pre-selected.
Fill the syringe up to the 10 mark
The syringe: a 1 ml U-100 insulin syringe (100 small marks = 1 ml), sold at any pharmacy.
Open this calculation in the calculatorThe protocol week
Timing is part of the protocol
The dose goes at night because the growth hormone pulse it stimulates occurs during sleep, and on an empty stomach because recent food —carbohydrates and fats in particular— blunts that release. Hence the minimum of 90 minutes after the last meal.
The reference week is five consecutive dosing days and two rest days, always the same ones. The rest is not optional: it is part of the documented pattern.
Documented precautions
- Reported effects: injection-site irritation, muscle pain, headache, fluid retention, carpal tunnel symptoms.
- It can raise glucose: monitoring is advised in profiles with insulin resistance or type 2 diabetes risk.
- Contraindicated with a history of pituitary tumors or pituitary surgery, active malignancy, mannitol allergy, pregnancy, and breastfeeding.
Documented interaction with tirzepatide: tesamorelin tends to raise glucose and tirzepatide to lower it; combining them produces unpredictable control and can reduce the efficacy of both. Review the Tirzepatide protocol if you work with both.
In our catalog
The 10 mg vial is on the Tesamorelin product page. In documented research practice it is combined with Ipamorelin + CJC-1295 for synergistic GH elevation, with GHK-Cu in aesthetic lines, and with MOTS-c in metabolism.
Frequently asked questions
How far does the 10 mg vial go?
10 complete doses of 1 mg —two weeks of the standard 5-day protocol— or 5 doses of 2 mg. A full 12-week cycle at 1 mg daily requires 6 vials.
Why at night and on an empty stomach?
The growth hormone pulse that tesamorelin stimulates occurs during sleep, and recent food blunts it. Dosing at night, at least 90 minutes after eating, respects both conditions.
Is a 4-week cycle useful as a trial?
The documentation agrees that it is not: tesamorelin's effect is cumulative, and cycles under 12 weeks show no tangible results. Starting this compound means committing to the full cycle.
What do I do if the vial gels during reconstitution?
Do not shake it or force it with heat. Let it rest and swirl it gently; if the gel or clumps persist, message us on WhatsApp with a photo before using it and we will review it with you.
Reference protocols compiled from documented research practice and adapted to our formats. Research-context documentation (RUO): not medical advice, not a prescription, and no substitute for professional evaluation.