Reference protocol: NAD+
With NAD+ the weekly load is the same across the three schedules —210 mg— and what you choose is the split: more gentle injections or fewer, more felt ones (it is an acidic compound and you notice it). To build tolerance, use half the dose of your schedule for the first two weeks.
Our schedule for NAD+ is 210 mg per week, and you choose how to split it: 30 mg daily —18 units at 166.7 mg/ml—, 70 mg three times a week —42 units— or 105 mg twice —63 units—. Always in the morning, never exceeding 3,000 mg per month; cycles of up to 12 weeks.
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30 mg every morning (18 U) — the gentlest dose, ideal if NAD+ stings you.
First time? Start here
Your NAD+ vial arrives with the compound as a dry powder. Before using it you mix it with a special water — that step is called reconstitution — and measure each dose with an insulin syringe. Here is everything you need, in order.
- Get what you need: your vial, a vial of bacteriostatic water (not included — it is the special mixing water), a 1 ml U-100 insulin syringe — ask for it exactly like that at any pharmacy — and alcohol wipes.
- Mix the powder with the water (reconstitute): the vial is never uncapped — the needle goes through the rubber stopper. Add 3 ml of water letting it run down the inside wall, then swirl gently — never shake; it turns clear in a few minutes (some compounds take longer). Measure the water with the same insulin syringe: full to the top is 1 ml — repeat until you complete the 3 ml. Once mixed, the vial goes in the fridge and lasts about 4 weeks; unmixed, also keep it refrigerated from the day it arrives.
- Fill the syringe up to the 18 mark: every small line on the insulin syringe is 1 unit (U). That mark equals the starting dose (30 mg); if your dose is different, pick it in the panel below and the number updates. And note: several protocols raise the dose after the first weeks — the dose steps further down tell you when your mark changes.
- Inject under the skin and you are done: belly area (two fingers away from the navel), skin cleaned with alcohol; pinch the skin, insert the full needle and inject slowly. Switch sides each time and use a new syringe for every injection. Used in the morning, on the schedule you choose: daily, 3 or 2 times a week.
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Quick dictionary — the words that stop beginners
- Reconstitute: mixing the vial's powder with the special water so it becomes a liquid you can measure.
- Bacteriostatic water: the special water for that mix (regular water will not do); sold separately — one vial covers several mixes.
- Unit (U): each small line on the insulin syringe. 100 units = 1 ml.
- U-100 syringe: the standard 1 ml insulin syringe with 100 marks; sold at any pharmacy.
- mg and mcg: milligrams and micrograms: 1 mg = 1,000 mcg. Mind the factor of one thousand when reading a dose.
- Subcutaneous: an injection under the skin (not into muscle or vein), usually in the belly area.
- Cycle and washout: using the product for a period and then stopping completely for some weeks; when resuming you start again from the starting dose.
Quick facts
| Parameter | Reference |
|---|---|
| Weekly total | 210 mg (house schedule) |
| Equivalent splits | 30 mg daily · 70 mg ×3/week · 105 mg ×2/week |
| Time of day | In the morning; a light meal can reduce adaptation effects |
| Tolerance | For the first 2 weeks, half the dose of your schedule |
| Monthly cap | 3,000 mg — do not exceed |
| Cycle | Up to 12 weeks · 4–6 week washout |
| Route | Subcutaneous (better tolerated) or intramuscular |
Three splits, the same weekly load
| Schedule | Dose | U-100 syringe | Weekly load |
|---|---|---|---|
| Daily | 30 mg | 18 U | 210 mg |
| 3 times a week | 70 mg | 42 U | 210 mg |
| Twice a week | 105 mg | 63 U | 210 mg |
The documented reference range sits between 50 and 100 mg per dose; the 105 mg of the twice-a-week schedule slightly exceeds it to keep the load even — we state it openly. If you prefer to stay within the documented range, use the daily or the 3-times-a-week split.
Whatever the split, use half the dose for the first two weeks: NAD+ is acidic and the body appreciates a gradual start. If a dose feels uncomfortable, halve it without guilt.
Reconstitution and syringe units
Our 500 mg vial is reconstituted with 3 ml of bacteriostatic water: it sits at 166.7 mg/ml.
The buttons follow the protocol's dose steps; the starting dose comes pre-selected.
Fill the syringe up to the 18 mark
The syringe: a 1 ml U-100 insulin syringe (100 small marks = 1 ml), sold at any pharmacy.
Open this calculation in the calculatorThe protocol week
See the full dose-to-units table
Units refer to an insulin U-100 syringe (100 units = 1 ml). Tap a figure to open it in the calculator. The last column shows how many complete doses the vial yields.
NAD+ is acidic, and the higher the concentration, the more it burns on injection. With our water vial (3 ml) it sits at 166.7 mg/ml; if the burning bothers you, a second water vial lets you go down to 100 mg/ml with 5 ml — the volume per dose grows, but the discomfort drops.
Subcutaneous or intramuscular
- Subcutaneous: slower, more even absorption, less painful, better tolerated. It can leave transient redness or nodules. It is the recommended starting route.
- Intramuscular: more pronounced effect but with a high risk of burning, muscle stiffness, and histamine-type reactions — flushing, chest tightness, throat tickle, cough. If they appear, go back to subcutaneous and dilute more.
Documented precautions
- Do not exceed 3,000 mg per month: in the documentation, excess is associated with liver and kidney damage.
- Do not use during pregnancy or breastfeeding, with active cancer, or while undergoing cancer treatment.
- Caution with diabetes (documented insulin resistance and elevated glucose), antihypertensives (it potentiates their effect), anticoagulants, and antidepressants.
- Quercetin and resveratrol multiply NAD+ levels and with them its adverse effects: flushing, nausea, itching, cramps, headache.
In our catalog
The 500 mg vial is on the NAD+ product page. In documented research practice it accompanies MOTS-c in mitochondrial lines, and as energy support in protocols with GLP agonists such as Tirzepatide.
Frequently asked questions
Why the same load across the three schedules?
Because 210 mg per week splits cleanly into 7, 3 or 2 doses (30, 70 and 105 mg): switching schedules changes neither your consumption nor how far the vial goes. You choose between more gentle injections or fewer, more felt ones — NAD+ stings more the larger the dose.
How far does the 500 mg vial go?
About two weeks on any of the three splits: 16 doses of 30 mg, 7 of 70 or 4 of 105. At 210 mg per week you use about 910 mg per month — under a third of the 3,000 mg cap.
Why in the morning?
NAD+ takes part in regulating circadian rhythm and cellular energy: dosed late it can interfere with sleep. The reference places it in the morning, with or without a light meal.
Does the injection hurt?
It is one of the peptides you feel the most because of its acidity, especially intramuscular. Subcutaneous and with more dilution the discomfort drops noticeably: if it burns, reconstitute with more water and review your technique — slowly, and rotating the site.
Reference protocols compiled from documented research practice and adapted to our formats. Research-context documentation (RUO): not medical advice, not a prescription, and no substitute for professional evaluation.